Journal article
Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy
AAK Moe, TG Bautista, MW Trewella, WS Korim, ST Yao, R Behrens, AK Driessen, AE McGovern, SB Mazzone
Iscience | CELL PRESS | Published : 2024
Abstract
In rats and guinea pigs, sensory innervation of the airways is derived largely from the vagus nerve, with the extrapulmonary airways innervated by Wnt1+ jugular neurons and the intrapulmonary airways and lungs by Phox2b+ nodose neurons; however, our knowledge of airway innervation in mice is limited. We used genetically targeted expression of enhanced yellow fluorescent protein-channelrhodopsin-2 (EYFP-ChR2) in Wnt1+ or Phox2b+ tissues to characterize jugular and nodose-mediated physiological responses and airway innervation in mice. With optical stimulation, Phox2b+ vagal fibers modulated cardiorespiratory function in a frequency-dependent manner while right Wnt1+ vagal fibers induced a sma..
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Awarded by Merck
Funding Acknowledgements
This study was supported by a grant (1078943) to S.B.M. from the National Health and Medical Research Council of Australia (NHMRC) , a grant (DP210103740) to S.B.M. from the Australian Research Council and a University of Melbourne early career researcher grant (1859105) to A.A.K.M. A.E.M. currently holds a Discovery Early Career Researcher Award (DE230101079) from the Australian Research Council. The authors would like to thank Dr Lincon Stamp and Dr Marlene Hao (Department of Anatomy and Physiology) for providing us with het-erozygous Wnt1-Cre and homozygous ChR2 mouse lines, Dr Phil Ryan (The Florey Institute of Neuroscience and Mental Health) for providing us with CGRP-Cre mouse line and Dr Ellie Cho (Biological Optical Microscopy Platform [BOMP] , The University of Melbourne) for her technical guidance on image processing in Fiji and members of BOMP.